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IFN-γ levels produced by lymphocytes stimulated by secreted-type proteins. ( A ) ELISA analysis of IFN-γ levels in splenocytes of immunized mice at different times (* P < 0.001 compared with MPT64 group). ( B ) Identification by the SDS-PAGE of MPT64. ( C ) WB identification of supernatant versus bacterial precipitate at different time points after MPT64 induction expression.

Journal: Microbiology Spectrum

Article Title: MPT64 antigen-induced immune responses as a novel diagnostic tool for tuberculosis

doi: 10.1128/spectrum.03381-24

Figure Lengend Snippet: IFN-γ levels produced by lymphocytes stimulated by secreted-type proteins. ( A ) ELISA analysis of IFN-γ levels in splenocytes of immunized mice at different times (* P < 0.001 compared with MPT64 group). ( B ) Identification by the SDS-PAGE of MPT64. ( C ) WB identification of supernatant versus bacterial precipitate at different time points after MPT64 induction expression.

Article Snippet: IFN-γ levels were measured using the human IFN-γ T-SPOT.TB kit (DAKEWE Biotechnology) and quantified as spot-forming units (SFUs) using an immunospot image analyzer (BioReader-E, BioSYS, Germany).

Techniques: Produced, Enzyme-linked Immunosorbent Assay, SDS Page, Expressing

The release of IFN-γ from both MPT64 and MPT64+CLE was much higher in active pulmonary TB patients than in the healthy control population. ( A ) IFN-γ levels of active tuberculosis and healthy control MPT64. ( B ) The ROC curve of the ELISpot for MPT64. ( C ) IFN-γ levels of active tuberculosis and healthy control MPT64+CLE. ( D ) The ROC curve of the ELISpot for MPT64+CLE. ( E ) Representative plot of MPT64 protein as antigen-stimulated PBMC in TB humans and healthy controls. ( F ) Representative plot of MPT64+CLE protein as antigen-stimulated PBMC in TB humans and healthy controls. “****” indicates a statistically significant difference.

Journal: Microbiology Spectrum

Article Title: MPT64 antigen-induced immune responses as a novel diagnostic tool for tuberculosis

doi: 10.1128/spectrum.03381-24

Figure Lengend Snippet: The release of IFN-γ from both MPT64 and MPT64+CLE was much higher in active pulmonary TB patients than in the healthy control population. ( A ) IFN-γ levels of active tuberculosis and healthy control MPT64. ( B ) The ROC curve of the ELISpot for MPT64. ( C ) IFN-γ levels of active tuberculosis and healthy control MPT64+CLE. ( D ) The ROC curve of the ELISpot for MPT64+CLE. ( E ) Representative plot of MPT64 protein as antigen-stimulated PBMC in TB humans and healthy controls. ( F ) Representative plot of MPT64+CLE protein as antigen-stimulated PBMC in TB humans and healthy controls. “****” indicates a statistically significant difference.

Article Snippet: IFN-γ levels were measured using the human IFN-γ T-SPOT.TB kit (DAKEWE Biotechnology) and quantified as spot-forming units (SFUs) using an immunospot image analyzer (BioReader-E, BioSYS, Germany).

Techniques: Control, Enzyme-linked Immunospot

Dominant epitopes in the T and B cells of the MPT64 protein. ( A ) ELISA measurement of IFN-γ release of each peptide and the complete sequence of MPT64. ( B ) The ELISA measurement of IFN-γ release from TB human PBMC stimulation by each peptide and the MPT64 complete sequence. ( C ) DTH of each polypeptide and the complete sequence of MPT64 in the mouse. ( D ) ELISA absorbance of the affinity of each polypeptide and the complete sequence of MPT64 to the MPT64 rabbit polyclonal antibody. ( E ) Spot hybridization shows the affinity of each polypeptide and the complete MPT64 sequence with the MPT64 rabbit polyclonal antibody. ( F ) Schematic diagram of the 3D predicted structure of each polypeptide, as well as the complete sequence of MPT64.

Journal: Microbiology Spectrum

Article Title: MPT64 antigen-induced immune responses as a novel diagnostic tool for tuberculosis

doi: 10.1128/spectrum.03381-24

Figure Lengend Snippet: Dominant epitopes in the T and B cells of the MPT64 protein. ( A ) ELISA measurement of IFN-γ release of each peptide and the complete sequence of MPT64. ( B ) The ELISA measurement of IFN-γ release from TB human PBMC stimulation by each peptide and the MPT64 complete sequence. ( C ) DTH of each polypeptide and the complete sequence of MPT64 in the mouse. ( D ) ELISA absorbance of the affinity of each polypeptide and the complete sequence of MPT64 to the MPT64 rabbit polyclonal antibody. ( E ) Spot hybridization shows the affinity of each polypeptide and the complete MPT64 sequence with the MPT64 rabbit polyclonal antibody. ( F ) Schematic diagram of the 3D predicted structure of each polypeptide, as well as the complete sequence of MPT64.

Article Snippet: IFN-γ levels were measured using the human IFN-γ T-SPOT.TB kit (DAKEWE Biotechnology) and quantified as spot-forming units (SFUs) using an immunospot image analyzer (BioReader-E, BioSYS, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Sequencing, Hybridization